Neutralizing antibody spectrum determines the antigenic profiles of emerging mutants of visna virus

Titration of Rinderpest Virus and Its Neutralizing Antibody

Selection of neutralizing antibody escape mutants with type A influenza virus HA-specific polyclonal antisera: possible significance for antigenic drift.

Ten antisera were produced in rabbits by two or three intravenous injections of inactivated whole influenza type A virions. All contained haemagglutination-inhibition (HI) antibody directed predominantly to an epitope in antigenic site B and, in addition, various amounts of antibodies to an epitope in site A and in site D. The ability of untreated antisera to select neutralization escape mutant...

Isolation of a herpes simplex virus-specific antigenic fraction which stimulates the production of neutralizing antibody.

Infection of mammalian cells with herpes simplex virus (HSV) results in the production of a number of virus-induced soluble antigens. Immunodiffusion analyses of the soluble antigen mixture (SAM) obtained from HSV-infected KB or BHK cells revealed at least six well-defined immunoprecipitin bands. Calcium phosphate chromatography (Brushite) was employed to separate one immunoprecipitin (designat...

Estimation of virus - neutralizing antibody in

Virus-neutralizing antibodies are seldom estimated in routine diagnostic laboratories because methods involving replicate series of tubed cell monolayers are so demanding of time and materials. Simplified methods for performing neutralization tests have been described, but these were generally developed for specific purposes and have required various forms of specialized apparatus. One of the e...

Estimation of virus-neutralizing antibody in microplates.

A simple and rapid method for the titration of virus-neutralizing antibody is described. The micro-apparatus and reagents are readily available from commercial sources. Results are included from selected cases which illustrate various conditions of immune status and response.